Protocol for preparing Drosophila genomic DNA to create chromosome-level de novo genome assemblies

AL Sperling, DK Fabian, E Garrison, DM Glover - STAR protocols, 2024

Abstract

De novo genome assemblies are common tools for examining novel biological phenomena in non-model organisms. Here, we present a protocol for preparing Drosophila genomic DNA to create chromosome-level de novo genome assemblies. We describe steps for high-molecular-weight DNA preparation with phenol or Genomic-tips, quality control, long-read nanopore sequencing, short-read DNA library preparation, and sequencing. We then detail procedures of genome assembly, annotation, and assessment that can be used for downstream comparison and functional analysis.

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