Dr Susana Sauret-Gueto
I manage all aspects for the smooth running of Research Services at CSC working with the CSC Research Support Team, and I am Deputy Safety Officer.
I prioritise supporting an inclusive and collaborative research culture. I am a senior researcher with a strong interest in influencing good research data management practices to produce high-quality research.
With broad experience in molecular genetics, synthetic biology, bioimaging, morphogenesis and plant development. With strong critical analytical thinking, throughout my career I’ve been exceptionally proactive to embrace new concepts and methods. As Microscopy Specialist, I manage the CSC Microscopy Facility and advice on Microscopy projects.
Waller M; Frangedakis E; Marron A; Sauret-Gueto S; Rever J; Raja Rubenstein Sabbagh C; Hibberd J; Haseloff J; Renzaglia K; Szövényi P The Plant Journal (2022) An optimised transformation protocol for Anthoceros agrestis and three more hornwort species bioRxiv 2022.08.10.503456; doi: https://doi.org/10.1101/2022.08.10.503456
Sauret-Güeto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, Markel K, Delmans M, West A, Patron NJ, Haseloff J. ACS Synth Biol. Systematic Tools for Reprogramming Plant Gene Expression in a Simple Model, Marchantia polymorpha. Synth Biol. 9(4):864-882 (2020). doi: 10.1021/acssynbio.9b00511.
Featured at Benchtalk: Sauret-Güeto S, Generating FAIR — Findable, Accessible, Interoperable, Reusable— Resources with Marchantia, a Prototype for Plant Synthetic Biology: https://www.benchling.com/2020/03/09/generating-fair-findable-accessible-interoperable-reusable-resources-with-marchantia-a-prototype-for-plant-synthetic-biology/
Sauret-Güeto S, Schiessl K., Bangham A., Sablowski R and Coen E. JAGGED controls Arabidopsis petal growth and shape by interacting with a divergent polarity field. PLoS Biol. 11(4): e1001550. doi:10.1371 journal.pbio.1001550 (2013). http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001548
Sauret-Güeto S, Grant C and Harberd N. Transient gibberellin application promotes Arabidopsis thaliana hypocotyl cell elongation without maintaining transverse orientation of microtubules on the outer tangential wall of epidermal cells. Plant J. 69:628-39 (2012). doi: 10.1111/j.1365-313X.2011.04817.x.
Flores-Pérez U, Sauret-Güeto S, Gas E, Jarvis P and Rodríguez-Concepción M. A mutant impaired in the production of plastome-encoded proteins uncovers a mechanism for the homeostasis of isoprenoid biosynthetic enzymes in Arabidopsis plastids. Plant Cell. 20: 1303-15 (2008). doi: 10.1105/tpc.108.058768
Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, Haseloff J. Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts. ACS Synth Biol. 10(7):1651-1666 (2021). doi: 10.1021/acssynbio.0c00637.
Frangedakis E, Markel K, Sauret-Gueto S, Haseloff J. Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts. Methods Mol Biol. 2317:343-365 (2021). doi: 10.1007/978-1-0716-1472-3_21. PMID: 34028781.
Crozet P, ….., Sauret-Gueto S, et al., Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 7(9):2074-2086 (2018). doi: 10.1021/acssynbio.8b00251.
Abley K, Sauret-Güeto S, Maree AFM, Coen E. Formation of polarity convergences underlying shoot outgrowths. eLife, 1;5. pii: e18165. doi: 10.7554/eLife.18165 (2016).
Pérez-Gil J, Urós AM, Sauret-Güeto S, Lois LM, Kirby J, Nishimoto M, Baidoo EEK, Keasling JD, Boronat A and Manuel Rodríguez-Concepción M. Mutations in Escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway. PLoS One 7(8): e43775. doi:10.1371/journal.pone.0043775 (2012).
Gas E, Flores-Pérez U, Sauret-Güeto S and Rodríguez-Concepción M. Hunting for plant nitric oxide synthase provides new evidence of a central role for plastids in nitric oxide metabolism. Plant Cell. 21:18-23 (2009).
Sauret-Güeto S., Botella-Pavía P, Flores-Pérez U, Martínez-García JF, San Román C, León P, Boronat A. and Rodríguez-Concepción M. Plastid cues post-transcriptionally regulate the accumulation of key enzymes of the methylerythritol phosphate pathway in Arabidopsis. Plant Phys. 141:75-84 (2006).
Sauret-Güeto S, Urós EM, Ibáñez E, Boronat A and Rodríguez-Concepción M. A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase. FEBS Lett. 580:736-740 (2006).
Besumbes O, Sauret-Güeto S, Phillips MA, Imperial S, Rodríguez-Concepción M and Boronat A. Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of Taxol. Biotechnol. Bioeng. 88: 168-75 (2004).
Sauret-Güeto S, Ramos-Valdivia A, Ibáñez E, Boronat A and Rodríguez-Concepción M. Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway. Biochem. Biophys. Res. Commun. 307: 408-15 (2003).
Rodríguez-Concepción M, Ahumada I, Diez-Juez E, Sauret-Güeto S, Lois LM, Gallego F, Carretero-Paulet L, Campos N and Boronat A. 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening. Plant J. 27: 213-222 (2001).
Campos, N, Rodríguez-Concepción M, Sauret-Güeto S, Gallego F, Lois LM and Boronat A. Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis. Biochem J. 353: 59-67 (2001).
